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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides and measurement of fluorescent intensity across the array indicates the abundance of a predetermined set of sequences. These sequences are typically specifically chosen to report on genes of interest within the organism’s genome.[1]
Fecha
Fuente Obra propio
Outor Thomas Shafee
Other versions

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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Fecha/OraMiniaturaDimenshonnanUsuarioKomentario
aktual12:22, 13 febrüari 2017Miniatura di e vershon di 12:22, 13 febrüari 20171,020 × 858 (67 KB)Evolution and evolvabilitytext as paths to avoid artefacts
08:40, 10 febrüari 2017Miniatura di e vershon di 08:40, 10 febrüari 20171,020 × 858 (66 KB)Evolution and evolvabilityReverted to version as of 05:08, 5 October 2016 (UTC)
08:40, 10 febrüari 2017Miniatura di e vershon di 08:40, 10 febrüari 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription.
05:08, 5 òktober 2016Miniatura di e vershon di 05:08, 5 òktober 20161,020 × 858 (66 KB)Evolution and evolvabilityUser created page with UploadWizard

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